-the sequence of amino acids in the polypeptide chain
-linked by peptide bonds
ii) Secondary Structure
-the folding of polypeptide chains into specific shape
- α -helix: coiled polypeptide chain
- β -pleated: folded polypeptide chain
-hydrogen bonds hold the peptide chains in place
-eg:keratin:present in hair and nails
iii. Tertiary structure
-protein is folded into a three-dimensional shape
-shape is stabilized by hydrogen bonds,hydrophobic intereactions,disulphide bridges and ionic bonds
-eg:enzyme,myoglobin
iv. Quartenary Structure
-more than one polypeptide chain
-forms a three dimensional shape
-hydrogen bonding,ionic bonds,hydrophobic interactions holds maintains its shape
-eg:haemoglobin,an oxygen carrying molecule which is made up of 2 α -helix and 2 β -pleated polypeptides,consists of 4 iron haem group for oxygen binding.
Structure of haemoglobin
Fibrous vs Globular Protein
Practice Essay Question
Discuss the importance of protein structure for determining enzyme specificity and its mode of action. (6)
Suggested Answer:
-enzyme is a globular protein
-each enzyme has precise amino acids sequence,tertiary structure
-hydrophilic R group outside the molecule-soluble in water
-active site of enzyme,catalyses chemical reactions has specific shape and functional groups to bind to substrate
-substrate can bind to active site of enzyme via non-covalent interactions;hydrogen bonding,hydrophobic interactions,Van der Waals interactions
-Changing the protein structure of enzyme will result in denaturation
Describe the pathway of amylase secretion in pancreatic cells. (8)
The question asks us to explain about PATHWAY,which is a process,from where to where.
1.We know amylase is an enzyme(globular protein) which is secreted by pancreatic cells.
2.To synthesise a protein,first we must have genetic information from DNA.
Now your knowledge about organelles comes in.
1. In nucleus,transcription occurs to synthesise mRNA strand.
2. mRNA then moves out from the nucleus and attaches on the ribosomes on the rough endoplasmic reticulum.
3. Protein is synthesised .and carried to Golgi apparatus in the form oftransporting vesicle.
4. The transporting vesicle fuses with the cis end of the Golgi body.
5. In Golgi body, the polypeptide chain is modified into glycoprotein by glycosylation.
6. A secretory vesicle containing amylase enzyme buds off the trans end of Golgi body.
7.The vesicle fuses with the plasma membrane and releases its content outside the pancreatic cell by exocytosis.
Why I differentiate the terms transporting and secretory vesicle?
Transporting vesicle is for transport within the cell itself.
Secretory vesicles is to transport proteins to be released from the cell.
Below is a diagram of what I have explained above.
This essay question integrates a few organelles that you have learnt in the form of a process,it requires you to think more besides only knowing the function of each organelles.
The very first chapter,you are more likely to find it in objective questions and very rarely in structured and essay questions.
Study the video below:
0:47 - Polarity 1:10 - Hydrogen Bonding 1:54 - Adhesion + Cohesion — movement up trees 2:30 - Surface Tension — Cohesion 2:54 - Solvent Properties — Polarity 3:57 - Density of Ice — Hydrogen Bonds 4:42 - Temperature Control — High Heat Capacity / High Specific Heat 5:28 - Temperature Control — Evaporation
Essay Practice Q: State the property of water that allows each of the following to take place and, in each case, explain its importance: a. the cooling of skin during sweating (3) b. the transport of glucose and ions in a mammal (3) c. much smaller temperature fluctuations in lake sand oceans than in terrestrial (land-based)habitats. (3) Explain the physical properties of water. (6)
Welcome to my blog. I'm Stephie and my blog will be mostly on Biology issues,especially STPM. Since working life is very busy, I will try to update the blog as frequent as I can.
The new STPM Biology is divided into 3 terms which I think is easier compared to the old format prior to STPM 2013 where you have to memorize your 1.5 years facts and go for the final exam. The good news is the new STPM Biology enables you to study for less than 10 chapters before your final exam in 0.5 years duration,so it's actually a bonus for you to study thoroughly and score better.
Term 1 focuses on biochemistry of cells.
Term 2 focuses on physiology.
Term 3 focuses on ecology and genetics.
Which term is the easiest and the hardest?
Term 1 is the easiest because the topics are lesser and more of what you have learnt in Form 4 and Form 5,basically an extension of some biological molecules,cell organelles,plasma membrane,enzymes,respiration and photosyntesis,so you should aim to score here.
Term 3 is the average paper,because there will be some calculations on population genetics and ecology where you can gain easy marks if you know the formulas well.
Term 2 can be said to be the hardest,many major and minor areas to focus,essay question can be from one of the minor area which you don't focus and you easily lose marks.So it's really time consuming to focus on this term.
What books do you need for STPM Biology?
I suggest you to use Sasbadi as your main reference for Term 1 and Term 2. For Term 3,you will need your teacher's help if he/she has the Longman 2012 VOL 1&2 you can photocopy some areas from these books. As there are new topics in the new STPM system,you will still need another book,either Oxford or Pelangi. Additional reference is the Campbell Biology if you're hardworking to read the facts and atttempt the questions on the CD,otherwise,it will be pointless to buy this book. Try searching for videos to facilitate your studying process,especially last 2 chapters in Term 1,first 6 chapters in Term 2 and some chapters on genetics in Term 3, as they are mainly complicated processes which you may not get the point at glance if you just study the facts blindly.
Scoring in Biology
Section A: This section consists of 15 multiple choice questions,1 mark each=15 marks. This is the No.1 helper,the questions are straight forward and I suggest you to practice the PYQ and from other sources you can find online or tuition. For an A,you will need to score at least 13/15 in this section.
Section B: This section consists of 2 structured questions which carry another 15 marks. The No.2 helper, Questions from this section are easy as well so the best score for this section is at least 13/15.
Section C: This section consists of 3 essay questions,15 marks each choose 2 out of 3 questions. This is the killer section. Many students do not study sufficiently for this section and lack application skills which make them lose plenty of marks. Don't study only the major areas of the chapter as many essay questions will include some marks from the minor part which you don't like to focus on.That way you will automatically lose marks if you cannot answer part of the question.Remember to read the question properly and don't misinterpret! If the question asks you to state differences, compare in a table form,this will ease the examiner to mark your paper because your points can be seen clearly.
Overall,STPM Biology is still considered easier compared to other papers such as Chemistry and Maths T. Don't look at the percentage of A's in the past,just focus and do more research on the topics that you have studied if it's your favourite subject. Pay attention to the learning outcomes,Practice more essay questions. Eventually your hard work will pay off.