酪酸はiPS細胞樹立を促進する

「エピジェネティックリモデリング・多能性関連遺伝子発現促進を介した酪酸によるヒトiPS細胞樹立改善 」に続き、ノースカロライナ大学のYi Zhangらのグループによっても、Butyrate（酪酸）がiPS細胞樹立を促進することを示した論文が発表されました。

J Biol Chem. 2010 Jun 16. [Epub ahead of print]

Butyrate promotes iPS cell generation.
Liang G, Taranova O, Xia K, Zhang Y.
http://www.ncbi.nlm.nih.gov/pubmed/20554530?dopt=Abstract

Recent studies have demonstrated that embryonic stem (ES) cell-like induced pluripotent stem (iPS) cells can be generated by enforced expression of defined transcription factors. The fact that cell fate change is accompanied with changes in epigenetic modifications prompted us to investigate whether chemicals known to modulate epigenetic regulators are capable of enhancing the efficiency of iPS generation. Here we report that butyrate, a natural small fatty acid and histone deacetylase inhibitor, significantly increases the efficiency of mouse iPS cell generation using the transcription factors Oct4, Sox2, Klf4 and c-Myc. We show that butyrate not only changes the reprogramming dynamics, but also increases the ratio of iPS cell colonies to total colonies, by reducing the frequency of partially reprogrammed cells and transformed cells. Detailed analysis reveals that the effect of butyrate on reprogramming appears to be mediated by c-Myc and occurs during an early stage of reprogramming. Genome-wide gene expression analysis revealed up-regulation of ES cell-enriched genes when MEFs are treated with butyrate. Thus, our study identifies butyrate as a chemical factor capable of promoting iPS cell generation.

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