日本のネットではノロウイルスは培養できないと言うのは常識のようだが
英語で調べたらあった！
Solving a 48-year-old mystery: scientists succeed at growing noroviruses in human intestinal cell cultures in the lab
Baylor College of MedicineBaylor College of Medicine NewsMolecular Virology and MicrobiologySolving a 48-year-old mystery: scientists succeed at growing noroviruses in human intestinal cell cultures in the lab
一部転載：
Human noroviruses – the leading viral cause of acute diarrhea around the world – have been difficult to study because scientists had not found a way to grow them in the lab. Now, more than 40 years after Dr. Albert Kapikian identified human noroviruses as a cause of severe diarrhea, scientists at Baylor College of Medicine have, for the first time, succeeded at growing noroviruses in laboratory cultures of human intestinal epithelial cells. This work, published today in Science, represents a major step forward in the study of human gastroenteritis viruses because it establishes a system in which a number of norovirus strains can be grown, which will allow researchers to explore and develop procedures to prevent and treat infection and to better understand norovirus biology.
“People have been trying to grow norovirus in the lab for a very long time. We tried for the last 20 years. Despite all the attempts and the success of growing other viruses, it remained a mystery why noroviruses were so hard to work with,” said senior author Dr. Mary Estes, Cullen endowed professor of human and molecular virology and microbiology at Baylor and emeritus founding director of the Texas Medical Center Digestive Diseases Center.
Noroviruses, also known as the cruise ship viruses, do not grow in laboratory cultures that traditionally support the growth of other viruses, such as transformed cells that are derived from cancerous tissues. In addition, noroviruses are species specific – human noroviruses only infect and cause disease in humans, and mouse noroviruses only do so in mice. Human noroviruses do not grow in mice or other small animal models typically used for research.
“My idea was that we had not succeeded at growing noroviruses because we didn’t have the right cell type,” said Estes. “We first showed that in patients with chronic norovirus infections, the virus could be detected in intestinal cells called enterocytes, but normal human enterocyte cells rapidly died when put into culture. A breakthrough came when we learned that Dr. Hans Clevers’ team in the Netherlands had developed a method to make a new type of human intestinal epithelial cell culture system including enterocytes. These novel, multi-cellular human cultures, called enteroids, are made from adult intestinal stem cells from patient tissues. We anticipated that putting the virus in these non-transformed human cell cultures would let the virus grow.”
“I have been working with Dr. Estes for five years trying to cultivate this virus,” said co-first author Dr. Khalil Ettayebi, senior staff scientist in Estes’s lab. “We tried many approaches but obtained only negative results until we started using the new human stem cell-derived enteroid cultures in the laboratory.”
It took Estes and colleagues about one year to get the human intestinal epithelial cultures growing well in the lab. Then, before they tested the cultures with noroviruses, they tested them with another human gastrointestinal virus, rotavirus.
“Rotavirus grew well in the human intestinal epithelial cell cultures,” said Estes. “Then, we tried the human norovirus and found that some strains would grow, but others wouldn’t. We suspected that still something was missing.”
The researchers tried to improve the growth of the viruses by adding to the cultures substances that are naturally present in the upper small intestine, the natural environment where the virus grows. Other intestinal viruses, such as rotavirus, use these substances to grow inside the body.
英語で調べたらあった！
Solving a 48-year-old mystery: scientists succeed at growing noroviruses in human intestinal cell cultures in the lab
Baylor College of MedicineBaylor College of Medicine NewsMolecular Virology and MicrobiologySolving a 48-year-old mystery: scientists succeed at growing noroviruses in human intestinal cell cultures in the lab
一部転載：
Human noroviruses – the leading viral cause of acute diarrhea around the world – have been difficult to study because scientists had not found a way to grow them in the lab. Now, more than 40 years after Dr. Albert Kapikian identified human noroviruses as a cause of severe diarrhea, scientists at Baylor College of Medicine have, for the first time, succeeded at growing noroviruses in laboratory cultures of human intestinal epithelial cells. This work, published today in Science, represents a major step forward in the study of human gastroenteritis viruses because it establishes a system in which a number of norovirus strains can be grown, which will allow researchers to explore and develop procedures to prevent and treat infection and to better understand norovirus biology.
“People have been trying to grow norovirus in the lab for a very long time. We tried for the last 20 years. Despite all the attempts and the success of growing other viruses, it remained a mystery why noroviruses were so hard to work with,” said senior author Dr. Mary Estes, Cullen endowed professor of human and molecular virology and microbiology at Baylor and emeritus founding director of the Texas Medical Center Digestive Diseases Center.
Noroviruses, also known as the cruise ship viruses, do not grow in laboratory cultures that traditionally support the growth of other viruses, such as transformed cells that are derived from cancerous tissues. In addition, noroviruses are species specific – human noroviruses only infect and cause disease in humans, and mouse noroviruses only do so in mice. Human noroviruses do not grow in mice or other small animal models typically used for research.
“My idea was that we had not succeeded at growing noroviruses because we didn’t have the right cell type,” said Estes. “We first showed that in patients with chronic norovirus infections, the virus could be detected in intestinal cells called enterocytes, but normal human enterocyte cells rapidly died when put into culture. A breakthrough came when we learned that Dr. Hans Clevers’ team in the Netherlands had developed a method to make a new type of human intestinal epithelial cell culture system including enterocytes. These novel, multi-cellular human cultures, called enteroids, are made from adult intestinal stem cells from patient tissues. We anticipated that putting the virus in these non-transformed human cell cultures would let the virus grow.”
“I have been working with Dr. Estes for five years trying to cultivate this virus,” said co-first author Dr. Khalil Ettayebi, senior staff scientist in Estes’s lab. “We tried many approaches but obtained only negative results until we started using the new human stem cell-derived enteroid cultures in the laboratory.”
It took Estes and colleagues about one year to get the human intestinal epithelial cultures growing well in the lab. Then, before they tested the cultures with noroviruses, they tested them with another human gastrointestinal virus, rotavirus.
“Rotavirus grew well in the human intestinal epithelial cell cultures,” said Estes. “Then, we tried the human norovirus and found that some strains would grow, but others wouldn’t. We suspected that still something was missing.”
The researchers tried to improve the growth of the viruses by adding to the cultures substances that are naturally present in the upper small intestine, the natural environment where the virus grows. Other intestinal viruses, such as rotavirus, use these substances to grow inside the body.