To analyze the aftereffect of Humifuse Euphorbia Herb Extract ( HEH) on abating insulin attrition in blazon 2 diabetic KK-Ay mice. Totally 40 KK-Ay mice fed with high-fat diet were disconnected into four groups: the metformin group, the archetypal group, the HEH low-dose accumulation and the HEH high-dose group, and orally administrated with metformin hydrochloride (250 mg x kg(-1)), distilled water, Humifuse Euphorbia Herb Extract 1 g x kg(-1) and 2 g x kg(-1).
Besides, C57BL/6J mice with accustomed augment were taken as the accustomed ascendancy accumulation and orally administrated with according distilled water. The articulate administering for the 5 groups lasted for eight weeks. Before and afterwards the experiment, weight, abnegation glucose and insulin altruism were determined. The morphological changes in pancreas were empiric through hematoxylin-eosin (HE) staining on pancreatic tissue sections. The serum insulin, TNF-α, IL-6, adiponectin (ADPN) and leptin (LEP) were detected by ELISA.
The after-effects showed that HEH could abate weight and abnegation glucose in KK-Ay mice, allay hyperinsulinemia, abate claret glucose-time AUC, access 30-min claret glucose abatement rate, abate insulin resistance, decidedly alleviate the pathomorphological changes in pancreas in anniversary group, abatement serum TNF-α, IL-6 and leptin levels in KK-Ay mice and acceleration serum ADPN level. This abstraction accepted that Humifuse Euphorbia Herb Extract www.botanical-extracts.net/Other/1046/ can alleviate the insulin attrition in KK-Ay mice, and its apparatus may be accompanying to the aftereffect on anarchic factors and adipocytokines.