アーティクル論文です。
STAP細胞の初期化の質に触れています。STAP細胞は、ES並みの初期化ではない！、というよりES細胞の持つ初期化状態とは、STAP細胞は異なる状態が書かれています。
酸に数分つけると、細胞がESなみの状態になると考えない人の方が、科学界には多いと思います。むしろ、ES細胞の初期化とは、又、別の細胞変化とらえて、研究されていく道があります。
デイ３とデイ７を比べて、デイ３で出ていた血液由来細胞の持つ遺伝子がデイ７で消失し、初期化遺伝子に入れ替わる様を見ていた科学者たちは、生命の不思議にふれたのでしょう
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Onday7,theOct4-GFP1spheres expressed pluripotency-related marker proteins22 (Oct4, SSEA1, Nanog and E-cadherin; Fig. 2a) and marker genes (Oct4,Nanog, Sox2, Ecat1 (also called Khdc3), Esg1 (Dppa5a),Dax1(Nrob1)andRex1(Zfp42);Fig.2 band Extended Data Fig.3a) in a manner comparable to those seen in ES cells24. Moderate levels of expression of these pluripotency marker genes were observed on day 3
(Fig.2 band ExtendedDataFig.3b). Notably, the Oct4-GFP cells on day 3, but not on day7, expressed early haematopoietic markergenes such as Flk1 (also called Kdr) and Tal1 (Extended DataFig.3c), indicating that Oct4-GFP cells on day3, as judged by their expression pattern at the population level, were still in a dynamic process of conversion. On day7, unlike CD451 cells and like ES cells, low-pH-induced Oct4GFP cells displayed extensive demethylation at the Oct4 and Nanog promoter areas (Fig.2c), indicating tha these cells underwent a substantial reprogramming of epigenetic status in these keygenes forpluripotency.
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STAP cellscompared to ES cells
STAP cells, unlikemouse ES cells, showed a limited capacity for self-renewal in theLIF-containing medium and did not efficiently form colonies in dissociationculture (Fig. 2f, g), even in the presence of the ROCK inhibitor Y-27632,which suppresses dissociation-induced apoptosis28,29 (Fig. 2h).
Also, even underhigh-density culture conditions after partial dissociation (Fig. 2i), STAP cell numbers started to decline substantially after two passages.
Furthermore, expression of the ES cell marker protein Esrrb was low in STAP cells (Extended DataFig. 5d, e). In general, female ES cells do not show X-chromosomal inactivation30 and contain no H3K27me3-dense foci (indicative of inactivated X chromosomes), unlike female CD45 cells and EpiSCs. In contrast, H3K27me3-dense foci were foundin, 40% of female STAP cells strongly positive for Oct4-GFP (Extended Data Fig.5f, g). STAP cells were also dissimilar to mouse EpiSCs, another category of pluripotentstem cell 21,22,29,31,and were positive for Klf4 and negative for the epithelialtight junction marker sclaudin7 and ZO-1 (Extended Data Fig. 5d, e).
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