【Heat 1】
1.Novlene Williams-Mills（JAM)49.88
2.Amantle Montsho（BOT)49.89
3.Anastasiya Kapachinskaya（RUS)50.30
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4.Aliann Pompey（GUY)50.71
5.Jessica Beard（USA)51.20
6.Norma González（COL)51.91
7.Kineke Alexander(VIN)53.43
DNF
Amaka Ogoegbunam（NGR)
【Heat 2】
1.Shericka Williams(JAM)49.51
2.Antonina Krivoshapka(RUS)49.67
3.Debbie Dunn(USA)49.95
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4.Nicola Sanders(GBR)50.45
5.Amy Mbacke Thiam(SEN)51.70
6.Folasade Abugan(NGR)51.75
7.Joy Nakhumicha Sakari(KEN)52.69
8.Solen Désert-Mariller(FRA)53.26
【Heat 3】
1.Sanya Richards(USA)50.21
2.Christine Ohuruogu(GBR)50.35
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3.Lyudmila Litvinova(RUS)50.52
4.Libania Grenot(ITA)50.85
5.Indira Terrero(CUB)51.87
6.Sorina Nwachukwu(GER)51.98
7.Tiandra Ponteen(SKN)53.22
8.Christine Day(JAM)53.46