Good morning!
I want to be the kind of person who doesn't give in to the wind or rain, eats curry bread from 7-Eleven in the morning, eats Grandmother Curry at Coco Ichibanya at night and draws the lottery for a limited edition spoon, drinks more alcohol than a Russian, and eats more curry than an Indian or Sri Lankan.
I'm a tooth-cavity angel!
First, I'd like to write down something I noticed after writing the previous article.
The lifespan of a Mini-Earth is roughly proportional to the size of the container, but if you think about it carefully, if the density of the aquatic plants is constant, the density of the allelochemicals should be the same, so it's unnatural for it to last longer if the container is larger.
This "Biocube" is the first time that we've deliberately exposed the Mini-Earth to direct sunlight and kept it at a constant temperature with air conditioning.
"Direct sunlight" and "no winter" seem to be fairly big factors, and we can't make a simple comparison with past Mini-Earths.
Now, I'll introduce some allelopathy research by professionals.
Well, I've only read the abstract.
This is a study that confirmed that Elodea can inhibit a type of blue-green algae.
This study was done in 1994, so you may be able to find more advanced research.
This study suggests that Elodea canonica's allelochemicals are easily degradable substances.
Experimental study on the inhibitory effect of large aquatic plants on phytoplankton proliferation
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06680532/
As a measure to inhibit abnormal proliferation of phytoplankton, there is a method that uses physiologically active substances metabolized by large aquatic plants.
Since these physiologically active substances are derived from living organisms, it is expected that even if they are actually used, there will be almost no impact on the ecosystem.
Therefore, in this study, as a basic study of a method to inhibit phytoplankton proliferation using physiologically active substances, physiologically active substances were extracted from large aquatic plants using methanol...
kaken.nii.ac.jp
This is from 2012: approximately 660 species of plants were surveyed, and 18 new allelochemicals were identified.
This shows how difficult it is to identify allelochemicals.
Biotechnology Research Advancement Center: Innovation Creation Basic Research Promotion Project NARO
https://www.naro.go.jp/laboratory/brain/inv_up/epf_report/2012/047998.html
NARO is an organization that conducts research and development on food, agriculture, and rural areas.
www.naro.go.jp
This is the latest research that has not yet been finalized.
It shows that carnosic acid, the main allelochemical in rosemary, is metabolized by soil microorganisms.
Biological conversion of allelochemicals by soil microorganisms and its effect on phytotoxicity
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20F20398/
Allelopathy is a phenomenon in which plants exert an inhibitory effect on the growth and development of surrounding plants through the production and release of biologically active substances.
A significant relationship has been found between plant growth in the presence of allelochemicals and soil microorganisms, but the details are unknown.
In this study, we investigated carnosic acid, the main allelochemical in rosemary (Rosmarinus officinalis)…
kaken.nii.ac.jp
If we expand this story to underwater topics and interpret it in a very convenient way, I think it means that in a mini-earth with a solid bottom sand like our "Biocube," there is a high possibility that microorganisms that break down allelochemicals are occurring.
Furthermore, if we combine this with the story that plant roots activate soil microorganisms, which I mentioned earlier, in a way that suits us, it seems that our "Biocube" has a very excellent mechanism.
This is a story about how Elodea canadensis is suppressed by the allelopathy of Azolla.
It seems to be a research project by high school students in 2011.
https://ous.repo.nii.ac.jp/record/3393/files/Naturalistae15-49-55.pdf
I have introduced the results of various searches, but probably the research on allelopathy is still not very advanced especially when it comes to allelopathy of aquatic plants.
Even more so when it comes to research on how it affects protozoans, fish, shrimp, etc.
That's my current understanding.
If anyone out there knows more about this, I'd love to hear any advice or corrections to my misunderstandings!
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