I will review PKC again.
PKCs are classified into conventional, novel, atypical PKC.
Conventional PKC consists of alpha, beta, gamma.
Novel PKC is comprised of delta, epsilon, eta, theta.
Atypical PKC comprises of zeta, iota, and PKM.
Conventional PKC is regulated by DAG and Ca.
Novel PKC is regulated by only DAG.
atypical PKC is regulated by neither Ca and DAG, but PS.
Effector proteins of conventional/Novel PKCs are MARCKS/c-Raf/IKK.
MARCKS basically binds actin and plasma membrane. When MARCKS is phosphorylated,
MARCKS detached from plasma membrane, leading to actin reorganization.
c-Raf conveys MEF/ERK/AP-2, MAP kinase, leading to cell proliferation/differentiaion.
IKK is upstream of IKb/NFkB, leading to activation of transcription.
Another effectors is like chimaerin, RasGRP, protein kinase D, Munc-13, and DGK.
RasGRP1 is GEF of Ras, this means that DAG regulates Ras signalling.
Chimaerin is a GAP of Rac, leading to gliomas and breast cancer.
Chimaerin is GAP of Rac.
Rac--PAK--LIMK--cofilin--actin
PKD is localized at Golgi appratus. PKD inhibition leads to abnormal Golgi structure.
This indicates that PKD is crucial for Golgi function.(by Malhotra)
Munc-13 is localized at the presynaptic side of synapses, which is related to neuronal plasticity.
DGK phosphorylates DAG, generating PA. PA also play important roles in a variety of cell functions.
In the case of cell motility, cdc42 and Rac make filopodia and lamellipodia at the leading edge.
In contrast, Rho make stress fiber at the back-end.
Rho--ROCK--LIM--cofilin--actin
Rho--mDia--actin
Rac-PAK-LIMK--cofilin--actin ramellipodia leading edge
cdc42--PAK--LIMK--cofilin--actin filopodia leading edge
cdc42--WASP--Arp2/3--actin
はっぴぃーはっぴぃー
Here is endogeneous review session.
We are always make a plan, and do something along the plan.
However, what persentage of plans were completed and followed though?
I try to make a plan that is impossible to do it.
Rather, it is very important to make a plan I can really do.
Otherwise, it is just wast of time.
And I tend to do all thing at once.
I should think the assignment of work.
Best condition is the condition I always do something in each day.
Today, I did everything but next day I have nothing to do.
This way is not good.
Assignment is very important.
And priority is important.
Deadline is one week later.
Even though I finish the work within a day, It doesn't make a difference.
Last thing is to think the qualitiy of work
There is no final goal to make quality better.
I need to make a happy medium/compromise.
But at the same time, we need to make things better.
For example, grant.
I do every year. I get used to do it.
Now I can set the way that when should I start the grant, and when I finish, and when I should review at least 3days ago.
I want to emphasis this point.
The plan I made should be completed!