Establishment of a Highly-sensitive Anti-EphB2 Monoclonal Antibody Eb2Mab-3 for Flow Cytometry
R Ubukata, H Suzuki, M Hirose, H Satofuka, T Tanaka… - 2024
EphB2 is a member of the Eph family tyrosine kinase receptors. EphB2 binds to
ephrin-B1, ephrin-B2, and ephrin-B3, which are critical regulators of vascular and
nervous development through controlling cell migration and axon guidance. EphB2
is overexpressed in tumors, including glioma, breast cancer, hepatocellular
carcinoma, and malignant mesothelioma, and it functions as a tumor promoter.
Therefore, the development of monoclonal antibodies (mAbs) against EphB2 is …
ephrin-B1, ephrin-B2, and ephrin-B3, which are critical regulators of vascular and
nervous development through controlling cell migration and axon guidance. EphB2
is overexpressed in tumors, including glioma, breast cancer, hepatocellular
carcinoma, and malignant mesothelioma, and it functions as a tumor promoter.
Therefore, the development of monoclonal antibodies (mAbs) against EphB2 is …
| • | 引用: Antitumor activities of a defucosylated anti‑EpCAM monoclonal …  |
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[HTML] An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM
Q Zhao, X Hong, Y Wang, S Zhang, Z Ding, X Meng… - Communications Biology, 2024
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical
bottleneck, particularly for rare or fragile macromolecular assemblies and those
suffering from denaturation and particle orientation distribution issues related to air-
water interface. In this study, we develop and characterize an immobilized antibody-
based affinity grid (IAAG) strategy based on the high-affinity PA tag/NZ-1 antibody
epitope tag system. We employ Pyr-NHS as a linker to immobilize NZ-1 Fab on the …
bottleneck, particularly for rare or fragile macromolecular assemblies and those
suffering from denaturation and particle orientation distribution issues related to air-
water interface. In this study, we develop and characterize an immobilized antibody-
based affinity grid (IAAG) strategy based on the high-affinity PA tag/NZ-1 antibody
epitope tag system. We employ Pyr-NHS as a linker to immobilize NZ-1 Fab on the …
| • | 引用: Tailored placement of a turn-forming PA tag into the structured … |
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[PDF] Μελέτη της επίδρασης των αιμοπεταλίων, σε καλλιέργεια καρκινικών κυττάρων HepG2, in vitro
Χ Λώλη - 2024
Στο σημείο αυτό, θα ήθελα να ευχαριστήσω θερμά τον επιβλέποντα καθηγητή μου, κ.
Αλέξανδρο Τσελέπη, ομότιμο καθηγητή Βιοχημείας-Κλινικής Χημείας του Τμήματος
Χημείας του Πανεπιστημίου Ιωαννίνων, για την ανάθεση της μεταπτυχιακής αυτής
διατριβής αλλά και για την συνεχή καθοδήγηση καθ'όλη την διάρκεια εκπόνησης των
πειραμάτων, την συμπαράσταση και ενθάρρυνση αλλά και τις ουσιώδεις συμβουλές.
Αλέξανδρο Τσελέπη, ομότιμο καθηγητή Βιοχημείας-Κλινικής Χημείας του Τμήματος
Χημείας του Πανεπιστημίου Ιωαννίνων, για την ανάθεση της μεταπτυχιακής αυτής
διατριβής αλλά και για την συνεχή καθοδήγηση καθ'όλη την διάρκεια εκπόνησης των
πειραμάτων, την συμπαράσταση και ενθάρρυνση αλλά και τις ουσιώδεις συμβουλές.
| • | 引用: A cancer-specific monoclonal antibody recognizes the aberrantly … |
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