Maintenance of R-loop structures by phosphorylated hTERT preserves genome integrity
M Machitani, A Nomura, T Yamashita, M Yasukawa… - Nature Cell Biology, 2024
As aberrant accumulation of RNA–DNA hybrids (R-loops) causes DNA damage and
genome instability, cells express regulators of R-loop structures. Here we report that
RNA-dependent RNA polymerase (RdRP) activity of human telomerase reverse
transcriptase (hTERT) regulates R-loop formation. We found that the phosphorylated
form of hTERT (p-hTERT) exhibits RdRP activity in nuclear speckles both in
telomerase-positive cells and telomerase-negative cells with alternative lengthening …
genome instability, cells express regulators of R-loop structures. Here we report that
RNA-dependent RNA polymerase (RdRP) activity of human telomerase reverse
transcriptase (hTERT) regulates R-loop formation. We found that the phosphorylated
form of hTERT (p-hTERT) exhibits RdRP activity in nuclear speckles both in
telomerase-positive cells and telomerase-negative cells with alternative lengthening …
| • | 引用: Phosphorylation of hTERT at threonine 249 is a novel tumor …  |
 |
 |
 |
 |
[PDF] Recent advances in immunotherapy in cancer treatment
AA Patel - Cellular and Molecular Biology, 2024
Immunotherapy has emerged as a transformative approach in cancer treatment,
leveraging the body's own immune system to recognize and eradicate cancer cells.
This review provides an overview of the recent advances and aspects in
immunotherapy in cancer biology, from established therapies like checkpoint
inhibitors and CAR-T cells to emerging innovative approaches and the challenges
associated with their clinical translation.
leveraging the body's own immune system to recognize and eradicate cancer cells.
This review provides an overview of the recent advances and aspects in
immunotherapy in cancer biology, from established therapies like checkpoint
inhibitors and CAR-T cells to emerging innovative approaches and the challenges
associated with their clinical translation.
| • | 引用: Development of anti-human T cell immunoreceptor with Ig and ITIM … |
|
|
|
|
[HTML] Dynamic movement of the Golgi unit and its glycosylation enzyme zones
A Harada, M Kunii, K Kurokawa, T Sumi, S Kanda… - Nature Communications, 2024
Abstract Knowledge on the distribution and dynamics of glycosylation enzymes in the
Golgi is essential for better understanding this modification. Here, using a
combination of CRISPR/Cas9 knockin technology and super-resolution microscopy,
we show that the Golgi complex is assembled by a number of small 'Golgi units' that
have 1-3 μm in diameter. Each Golgi unit contains small domains of glycosylation
enzymes which we call 'zones'. The zones of N-and O-glycosylation enzymes are …
Golgi is essential for better understanding this modification. Here, using a
combination of CRISPR/Cas9 knockin technology and super-resolution microscopy,
we show that the Golgi complex is assembled by a number of small 'Golgi units' that
have 1-3 μm in diameter. Each Golgi unit contains small domains of glycosylation
enzymes which we call 'zones'. The zones of N-and O-glycosylation enzymes are …
| • | 引用: PA tag: a versatile protein tagging system using a super high … |
|
|
|
|
[HTML] Isocitrate Dehydrogenase Inhibitors in Glioma: From Bench to Bedside
MH Ser, M Webb, A Thomsen, U Sener - Pharmaceuticals, 2024
Isocitrate dehydrogenase (IDH) mutant gliomas are a primary malignancy of the
central nervous system (CNS) malignancies, most commonly affecting adults under
the age of 55. Standard of care therapy for IDH-mutant gliomas involves maximal
safe resection, radiotherapy, and chemotherapy. However, despite good initial
responses to multimodality treatment, recurrence is virtually universal. IDH-mutant
gliomas represent a life-limiting prognosis. For this reason, there is a great need for …
central nervous system (CNS) malignancies, most commonly affecting adults under
the age of 55. Standard of care therapy for IDH-mutant gliomas involves maximal
safe resection, radiotherapy, and chemotherapy. However, despite good initial
responses to multimodality treatment, recurrence is virtually universal. IDH-mutant
gliomas represent a life-limiting prognosis. For this reason, there is a great need for …
| • | 引用: Quantitative metabolome analysis profiles activation of … |
|
|
|
|
[HTML] 7 Tesla magnetic resonance spectroscopic imaging predicting IDH status and glioma grading
C Cadrien, S Sharma, P Lazen, R Licandro, J Furtner… - Cancer Imaging, 2024
Introduction With the application of high-resolution 3D 7 Tesla Magnetic Resonance
Spectroscopy Imaging (MRSI) in high-grade gliomas, we previously identified
intratumoral metabolic heterogeneities. In this study, we evaluated the potential of 3D
7 T-MRSI for the preoperative noninvasive classification of glioma grade and
isocitrate dehydrogenase (IDH) status. We demonstrated that IDH mutation and
glioma grade are detectable by ultra-high field (UHF) MRI. This technique might …
Spectroscopy Imaging (MRSI) in high-grade gliomas, we previously identified
intratumoral metabolic heterogeneities. In this study, we evaluated the potential of 3D
7 T-MRSI for the preoperative noninvasive classification of glioma grade and
isocitrate dehydrogenase (IDH) status. We demonstrated that IDH mutation and
glioma grade are detectable by ultra-high field (UHF) MRI. This technique might …
| • | 引用: Quantitative metabolome analysis profiles activation of … |