[HTML] Combining RNAscope, Immunohistochemistry (IHC) and Digital Image Analysis to Assess Podoplanin (PDPN) Protein and PDPN_mRNA Expression on Formalin …
LC Tomescu, AA Cosma, MP Fenesan, E Melnic… - Current Issues in Molecular …, 2024
The expression and function of podoplanin (PDPN) in the normal human placenta
has been debated in placental evaluation. This study emphasizes the importance of
a multimodal approach of PDPN expression in normal human placentas. A complete
examination is performed using immunohistochemistry, RNAscope and automated
Digital Image examination (DIA) interpretation. QuPath DIA-based analysis
automatically generated the stromal and histological scores of PDPN expression for …
has been debated in placental evaluation. This study emphasizes the importance of
a multimodal approach of PDPN expression in normal human placentas. A complete
examination is performed using immunohistochemistry, RNAscope and automated
Digital Image examination (DIA) interpretation. QuPath DIA-based analysis
automatically generated the stromal and histological scores of PDPN expression for …
| • | 1 件目の引用Immunohistochemical Analysis Using Monoclonal …  |
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[PDF] CD44 and its implication in neoplastic diseases
Y Xu, Z Bai, T Lan, C Fu, P Cheng - MedComm, 2024
CD44, a nonkinase single span transmembrane glycoprotein, is a major cell surface
receptor for many other extracellular matrix components as well as classic markers of
cancer stem cells and immune cells. Through alternative splicing of CD44 gene,
CD44 is divided into two isoforms, the standard isoform of CD44 (CD44s) and the
variant isoform of CD44 (CD44v). Different isoforms of CD44 participate in regulating
various signaling pathways, modulating cancer proliferation, invasion, metastasis …
receptor for many other extracellular matrix components as well as classic markers of
cancer stem cells and immune cells. Through alternative splicing of CD44 gene,
CD44 is divided into two isoforms, the standard isoform of CD44 (CD44s) and the
variant isoform of CD44 (CD44v). Different isoforms of CD44 participate in regulating
various signaling pathways, modulating cancer proliferation, invasion, metastasis …
| • | 引用: A defucosylated anti‑CD44 monoclonal antibody 5‑mG2a‑f exerts … |
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Novel Near-Infrared Fluorescent Probe for Hepatocyte Growth Factor in Vivo Imaging in Surgical Navigation of Colorectal Cancer
S Chen, G Li, R Pan, K Zhou, W Wen, J Tao, F Wang… - Analytical Chemistry, 2024
Despite recent advancements in colorectal cancer (CRC) treatment, the prognosis
remains unfavorable primarily due to high recurrence and liver metastasis rates.
Fluorescence molecular imaging technologies, combined with specific probes, have
gained prominence in facilitating real-time tumor resection guided by fluorescence.
Hepatocyte growth factor (HGF) is overexpressed in CRC, but the advancement of
HGF fluorescent probes has been impeded by the absence of effective HGF …
remains unfavorable primarily due to high recurrence and liver metastasis rates.
Fluorescence molecular imaging technologies, combined with specific probes, have
gained prominence in facilitating real-time tumor resection guided by fluorescence.
Hepatocyte growth factor (HGF) is overexpressed in CRC, but the advancement of
HGF fluorescent probes has been impeded by the absence of effective HGF …
| • | 引用: Macrocyclic peptide-based inhibition and imaging of hepatocyte … |
Current research insights into the role of CTLA‐4 in hepatitis B virus (HBV) infection
Q Gu, S Yin, X Tong, F Rui, Y Zhu, X Ma, R Huang… - Journal of Viral Hepatitis
Chronic hepatitis B virus (HBV) infection is a significant global public health concern,
and the clearance of HBV is closely linked to the activity of HBV‐specific T cells,
which is regulated by various co‐suppressor molecules. Cytotoxic T lymphocyte‐
associated antigen 4 (CTLA‐4) is among these co‐suppressor molecules which
induces T cell exhaustion by competitively inhibiting CD28 and dampening the
function of HBV‐specific T cells. CTLA‐4 also plays a role in the regulation of T …
and the clearance of HBV is closely linked to the activity of HBV‐specific T cells,
which is regulated by various co‐suppressor molecules. Cytotoxic T lymphocyte‐
associated antigen 4 (CTLA‐4) is among these co‐suppressor molecules which
induces T cell exhaustion by competitively inhibiting CD28 and dampening the
function of HBV‐specific T cells. CTLA‐4 also plays a role in the regulation of T …
| • | 引用: Prostaglandin E2-induced immune suppression via cytotoxic T … |
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Circulating tumor cell detection may offer earlier diagnosis in patients suspected of asbestos-related lung cancer
H Ke, S Kao, N van Zandwijk, JEJ Rasko, D Yeo - Lung Cancer
Abstract Asbestos-Related Lung Cancer (ARLC) presents ongoing diagnostic
challenges despite improved imaging technologies. The long latency period,
coupled with limited access to occupational/environmental data, confounding effects
of smoking and other carcinogens, adds complexity to the diagnostic process.
Compounding these challenges is the absence of a specific histopathologic or
mutational signature of ARLC. A correlation between PD-L1 expression and …
challenges despite improved imaging technologies. The long latency period,
coupled with limited access to occupational/environmental data, confounding effects
of smoking and other carcinogens, adds complexity to the diagnostic process.
Compounding these challenges is the absence of a specific histopathologic or
mutational signature of ARLC. A correlation between PD-L1 expression and …
| • | 引用: Novel circulating tumor cell‑detection chip combining conventional … |
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[HTML] VEGF-C and 5-Fluorouracil Improve Bleb Survival in a Rabbit Glaucoma Surgery Trabeculectomy Model
J Wu, L Zhou, Y Liu, X Zhang, Y Yang, X Zhu, Q Bu… - … Ophthalmology & Visual …, 2024
Purpose: To evaluate VEGF-C–induced lymphoproliferation in conjunction with 5-
fluorouracil (5-FU) antimetabolite treatment in a rabbit glaucoma filtration surgery
(GFS) model. Methods: Thirty-two rabbits underwent GFS and were assigned to four
groups (n= 8 each) defined by subconjunctival drug treatment:(a) VEGF-C combined
with 5-FU,(b) 5-FU,(c) VEGF-C,(d) and control. Bleb survival, bleb measurements,
and IOP were evaluated over 30 days. At the end, histology and anterior segment …
fluorouracil (5-FU) antimetabolite treatment in a rabbit glaucoma filtration surgery
(GFS) model. Methods: Thirty-two rabbits underwent GFS and were assigned to four
groups (n= 8 each) defined by subconjunctival drug treatment:(a) VEGF-C combined
with 5-FU,(b) 5-FU,(c) VEGF-C,(d) and control. Bleb survival, bleb measurements,
and IOP were evaluated over 30 days. At the end, histology and anterior segment …
| • | 引用: Functional, structural, and molecular identification of lymphatic … |
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Molecular simulation screening and sensory evaluation unearth a novel kokumi compound with bitter-masking effect: N-lauroyl-L-tryptophan
L Cai, L Li, X Zhao, L Wang, Y Cheng, W Gao, C Cui - Food Chemistry, 2024
Abstract N-lauroyl-L-tryptophan (LT), which has the strongest potential flavor-
presenting activity, was skillfully screened from numerous N-Lau-AAs docked to
different taste receptors by molecular simulation techniques. Subsequently, LT was
synthesized employing food-grade commercial enzymes and structurally
characterized, the optimized yields of LT could reach 69.08%, 76.16%, and 50.40%,
respectively. Sensory and E-tongue evaluations showed that LT at 1 mg/L …
presenting activity, was skillfully screened from numerous N-Lau-AAs docked to
different taste receptors by molecular simulation techniques. Subsequently, LT was
synthesized employing food-grade commercial enzymes and structurally
characterized, the optimized yields of LT could reach 69.08%, 76.16%, and 50.40%,
respectively. Sensory and E-tongue evaluations showed that LT at 1 mg/L …
| • | 引用: Structural basis for perception of diverse chemical substances by … |
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Impact of the Siglec-Sialoglycan Axis on the Cancer Immune Response
MS Ahmad, S Seriah, SS Siddiqui - 2024
Sialic acid-binding immunoglobulin-like lectins (Siglecs) have heavily mediated the
tumour landscape and shown significant deregulation in many tumour malignancies
by facilitating tumour progression and metastasis; such sialoglycan ligands have
also contributed to tumour development. Primarily, the Siglec/sialoglycan axis is
capable of modulating immunosuppression and signalling pathways, preventing
myeloid cell activation and subsequent generation of an immune response. This …
tumour landscape and shown significant deregulation in many tumour malignancies
by facilitating tumour progression and metastasis; such sialoglycan ligands have
also contributed to tumour development. Primarily, the Siglec/sialoglycan axis is
capable of modulating immunosuppression and signalling pathways, preventing
myeloid cell activation and subsequent generation of an immune response. This …
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