https://www.sigmaaldrich.com/
https://www.sigmaaldrich.com/
https://www.sigmaaldrich.com/
MABE1995
Anti-TERT Antibody, clone TwMab-1
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
antibody product type
primary antibodies
クローン
TwMab-1, monoclonal
分子量
calculated mol wt 127 kDa
observed mol wt ~127 kDa
精製方法
using protein G
species reactivity
human
包装
antibody small pack of 100
technique(s)
western blot: suitable
特異性
Clone TwMab-1 is a mouse monoclonal antibody that detects Telomerase reverse transcriptase (TERT). It targets an epitope within 11 amino acids from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 11 amino acids from the N-terminal half of human Telomerase reverse transcriptase (TERT).
アプリケーション
Quality Control Testing
Isotype testing: Identity confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1k.
Tested Applications
Western Blotting Analysis: A 1:100 dilution from a representative lot detected TERT in lysate from HEK293T cells with ectopic expression of hTERT (positive), but not in lysate from wild-type HEK293T cells (negative). ( Data courtesy of Dr. Kenkichi Masutomi, National Cancer Center Research Institute, Tokyo, Japan).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Isotype testing: Identity confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1k.
Tested Applications
Western Blotting Analysis: A 1:100 dilution from a representative lot detected TERT in lysate from HEK293T cells with ectopic expression of hTERT (positive), but not in lysate from wild-type HEK293T cells (negative). ( Data courtesy of Dr. Kenkichi Masutomi, National Cancer Center Research Institute, Tokyo, Japan).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Telomerase reverse transcriptase (UniProt: O14746; also known as EC:2.7.7.49, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2) is encoded by the TERT (also known as EST2, TCS1, TRT) gene (Gene ID: 7015) in human. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase responsible for adding TTAGGG repeats to the chromosome telomere ends. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. Cells with low or no telomerase expression lose telomere repeats during cell division, eventually resulting in cellular senescence. Most cancer cells, germ cells, and embryonic stem cells express high levels of telomerase, thus contributing to pluripotency and immortality. In addition to its telomere maintenance function, telomerase also has a pro-survival role in cellular resistance against DNA damage and ensuing apoptosis. Most cancer cells are highly proliferative and express high levels of nuclear telomerase activity. TERT is reported to shuttle between nuclear and cytoplasm and this shuttling is depended upon cell cycle, phosphorylation state, transformation, and DNA damage. TERT is also detected in the mitochondria where it helps prevent nuclear DNA damage by decreasing mitochondrial reactive oxygen species (ROS). Following exposure to H2O2 or -irradiation, cancer cells capable of excluding TERT from the nucleus display little or no DNA damage, while TERT nuclear retainment results in high DNA damage. Phosphorylation at tyrosine 707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its anti-apoptotic activity and its dephosphorylation by SHP2/PTPN11 leads to its nuclear retention. Phosphorylation at serine 227 by AKT is reported to promote nuclear location. Phosphorylation of TERT at threonine 249 is reported to be a novel tumor biomarker of aggressive cancer with poor prognosis. (Ref.: Masuda, Y., et al. (2022). J. Pathol. 257(2); 172-185; Jacob, S., et al. (2008). J. Biol. Chem. 283(48); 33155-33161; Haendeler, J., et al. (2004). Circ. Res. 94(6); 768-775).
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Stable for 1 year at +2°C to +8°C from date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
https://www.sigmaaldrich.com/JP/ja/product/mm/mabc1795
MABC1795
Anti-phospho-TERT (Thr249) Antibody, clone TpMab-3
別名:
EC:2.7.7.49, HEST2, TP2, Telomerase catalytic subunit, Telomerase reverse transcriptase, Telomerase-associated protein 2
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
antibody product type
primary antibodies
クローン
TpMab-3, monoclonal
分子量
calculated mol wt 127 kDa
observed mol wt ~127 kDa
精製方法
using protein G
species reactivity
human
包装
antibody small pack of 100
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
特異性
Clone TpMab-3 is a mouse monoclonal antibody that detects phospho-TERT (Thr249).
免疫原
KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphothreonine 249 from the N-terminal half of human Telomerase reverse transcriptase (TERT).
アプリケーション
Quality Control Testing
Isotype testing: Identity confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1k.
Tested Applications
Immunofluorescence Analysis: A representative lot detected TERT in Immunofluorescence applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Western Blotting Analysis: A 1:250 dilution from a representative lot detected TERT in Mitotic HeLa cells with RO3306 treatment (negative) versus mitotic HeLa cell samples (positive), where the positive control samples were obtained by IP using clone 10E9-2 to enrich hTERT ( Data courtesy of Dr. Kenkichi Masutomi, National Cancer Center Research Institute, Tokyo, Japan).
Western Blotting Analysis: A representative lot detected TERT in Western Blotting applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Immunohistochemistry Applications: A representative lot detected TERT in Immunohistochemistry applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Isotype testing: Identity confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1k.
Tested Applications
Immunofluorescence Analysis: A representative lot detected TERT in Immunofluorescence applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Western Blotting Analysis: A 1:250 dilution from a representative lot detected TERT in Mitotic HeLa cells with RO3306 treatment (negative) versus mitotic HeLa cell samples (positive), where the positive control samples were obtained by IP using clone 10E9-2 to enrich hTERT ( Data courtesy of Dr. Kenkichi Masutomi, National Cancer Center Research Institute, Tokyo, Japan).
Western Blotting Analysis: A representative lot detected TERT in Western Blotting applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Immunohistochemistry Applications: A representative lot detected TERT in Immunohistochemistry applications (Matsuda, Y., et al. (2022). J Pathol. 257(2):172-185).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Telomerase reverse transcriptase (UniProt: O14746; also known as EC:2.7.7.49, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2) is encoded by the TERT (also known as EST2, TCS1, TRT) gene (Gene ID: 7015) in human. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase responsible for adding TTAGGG repeats to the chromosome telomere ends. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. Cells with low or no telomerase expression lose telomere repeats during cell division, eventually resulting in cellular senescence. Most cancer cells, germ cells, and embryonic stem cells express high levels of telomerase, thus contributing to pluripotency and immortality. In addition to its telomere maintenance function, telomerase also has a pro-survival role in cellular resistance against DNA damage and ensuing apoptosis. Most cancer cells are highly proliferative and express high levels of nuclear telomerase activity. TERT is reported to shuttle between nuclear and cytoplasm and this shuttling is depended upon cell cycle, phosphorylation state, transformation, and DNA damage. TERT is also detected in the mitochondria where it helps prevent nuclear DNA damage by decreasing mitochondrial reactive oxygen species (ROS). Following exposure to H2O2 or -irradiation, cancer cells capable of excluding TERT from the nucleus display little or no DNA damage, while TERT nuclear retainment results in high DNA damage. Phosphorylation at tyrosine 707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its anti-apoptotic activity and its dephosphorylation by SHP2/PTPN11 leads to its nuclear retention. Phosphorylation at serine 227 by AKT is reported to promote nuclear location. Phosphorylation of TERT at threonine 249 is reported to be a novel tumor biomarker of aggressive cancer with poor prognosis. (Ref.: Masuda, Y., et al. (2022). J. Pathol. 257(2); 172-185; Jacob, S., et al. (2008). J. Biol. Chem. 283(48); 33155-33161; Haendeler, J., et al. (2004). Circ. Res. 94(6); 768-775).
物理的形状
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.