Development of a Sensitive Anti-Mouse CD39 Monoclonal Antibody (C39Mab-1) for Flow Cytometry and Western Blot Analyses
Y Okada, H Suzuki, MK Kaneko, Y Kato - Monoclonal Antibodies in Immunodiagnosis …, 2024
CD39 is involved in adenosine metabolism by converting extracellular ATP to
adenosine. As extracellular adenosine plays a critical role in the immune
suppression of the tumor microenvironment, the inhibition of CD39 activity by
monoclonal antibodies (mAbs) is one of the important strategies for tumor therapy.
This study developed specific and sensitive mAbs for mouse CD39 (mCD39) using
the Cell-Based Immunization and Screening method. The established anti-mCD39 …
adenosine. As extracellular adenosine plays a critical role in the immune
suppression of the tumor microenvironment, the inhibition of CD39 activity by
monoclonal antibodies (mAbs) is one of the important strategies for tumor therapy.
This study developed specific and sensitive mAbs for mouse CD39 (mCD39) using
the Cell-Based Immunization and Screening method. The established anti-mCD39 …
| • | 引用: Antitumor activities of a defucosylated anti‑EpCAM monoclonal …  |
[PDF] Nanosphere pharmacodynamics improves safety of immunostimulatory cytokine therapy
RA Lacinski, SA Dziadowicz, A Stewart… - iScience, 2024
Systemic administration of interleukin (IL)-12 induces potent anti-tumor immune
responses in preclinical cancer models through the systemic activation of effector
immune cells and release of proinflammatory cytokines. IL-12-loaded PLGA
nanospheres (IL12ns) are hypothesized to improve therapeutic efficacy and thwart
unwanted side effects observed in previous human clinical trials. Through the
investigation of peripheral blood and local tissue immune responses in healthy …
responses in preclinical cancer models through the systemic activation of effector
immune cells and release of proinflammatory cytokines. IL-12-loaded PLGA
nanospheres (IL12ns) are hypothesized to improve therapeutic efficacy and thwart
unwanted side effects observed in previous human clinical trials. Through the
investigation of peripheral blood and local tissue immune responses in healthy …
| • | 引用: Prostaglandin E2-induced immune suppression via cytotoxic T … |
Precision Medicine in Veterinary Science
E Chon, W Hendricks, M White, L Rodrigues… - Veterinary Clinics: Small …, 2024
The human cancer precision medicine paradigm has been built on the foundation of
understanding cancer's genomic underpinnings and great individual variability
Remarkable improvements in human cancer patient outcomes have been achieved
in recent decades, progress best represented by the dramatic drop in cancer death
rates. As of 2020, death rates have dropped by 33% relative to their peak in 1991,
accounting for 3.8 million lives saved. This progress is due to improvements in …
understanding cancer's genomic underpinnings and great individual variability
Remarkable improvements in human cancer patient outcomes have been achieved
in recent decades, progress best represented by the dramatic drop in cancer death
rates. As of 2020, death rates have dropped by 33% relative to their peak in 1991,
accounting for 3.8 million lives saved. This progress is due to improvements in …
| • | 引用: PD-L1 immunohistochemistry for canine cancers and clinical … |
[HTML] DNA mismatch repair system regulates the expression of PD-L1 through DNMTs in cervical cancer
F Guo, R Lu, W Kong, M Anwar, Y Feng - Cancer Cell International, 2024
Cervical cancer (CC) is a potential clinical application of PD-1/PD-L1 inhibitor. We
aimed to study the mechanism of DNA mismatch repair (MMR) system regulating the
expression of PD-L1 in CC through DNA methyltransferase (DNMTs). We collected
pathological specimens from 118 cases of CC to analyze the relationship between
PD-L1 expression and DNMTs in different MMR states. RNA interference (RNAi)
technique was used to simulate the formation of CC cell line with MMR deficiency …
aimed to study the mechanism of DNA mismatch repair (MMR) system regulating the
expression of PD-L1 in CC through DNA methyltransferase (DNMTs). We collected
pathological specimens from 118 cases of CC to analyze the relationship between
PD-L1 expression and DNMTs in different MMR states. RNA interference (RNAi)
technique was used to simulate the formation of CC cell line with MMR deficiency …
| • | 引用: Increase of cells expressing PD‐1 and PD‐L1 and enhancement of … |
C-terminal amino acids in the type I transmembrane domain of L-type lectin VIP36 affect γ-secretase susceptibility
Z Hua, R Watanabe, T Fukunaga, Y Matsui… - … and Biophysical Research …, 2024
Regulated intramembrane proteolysis (RIP) is a two-step processing mechanism for
transmembrane proteins consisting of ectodomain shedding (shedding), which
removes the extracellular domain through juxtamembrane processing and
intramembrane proteolysis, which processes membrane-anchored shedding
products within the transmembrane domain. RIP irreversibly converts one
transmembrane protein into multiple soluble proteins that perform various …
transmembrane proteins consisting of ectodomain shedding (shedding), which
removes the extracellular domain through juxtamembrane processing and
intramembrane proteolysis, which processes membrane-anchored shedding
products within the transmembrane domain. RIP irreversibly converts one
transmembrane protein into multiple soluble proteins that perform various …
| • | 引用: Mechanistic insights into ectodomain shedding: susceptibility of … |
[PDF] A change of rhodocytin's suprastructure turns the agonist into an antagonist of tumor cell induced platelet aggregation
WMJ Obermann, S König, MK Feickert, R Sanz‐Soler… - The FASEB Journal, 2024
Cancer cells circulating in the blood attach to platelets by direct cell–cell interactions
via several receptor‐counterreceptor contacts and indirectly by fibrin bridges which
connect the two cell types by distinct integrin receptors. In the microenvironment of
these tumor cell platelet aggregates (TCPAs), the tumor cells are shielded from the
shear stress of the blood flow and from attack by the immune system. This supports
hematogenous metastasis and tumor cell induced thrombosis. Platelet activation is …
via several receptor‐counterreceptor contacts and indirectly by fibrin bridges which
connect the two cell types by distinct integrin receptors. In the microenvironment of
these tumor cell platelet aggregates (TCPAs), the tumor cells are shielded from the
shear stress of the blood flow and from attack by the immune system. This supports
hematogenous metastasis and tumor cell induced thrombosis. Platelet activation is …
| • | 引用: A platform of C-type lectin-like receptor CLEC-2 for binding O … |
[PDF] A Biomimetic Synthetic Strategy can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns
Y Wu, G Bosman, D Chapla, C Huang, K Moremen… - 2024
Keratan sulfate (KS) is a proteoglycan that is widely expressed in the extracellular
matrix of various tissue types where it performs multiple biological functions. KS is
the least understood proteoglycan, which in part is due to a lack of panels of well-
defined KS oligosaccharides that are needed for structure-binding studies, as
analytical standards, to examine substrate specificities of keratinases and for drug
development. Here, we report a biomimetic approach that makes it possible to install …
matrix of various tissue types where it performs multiple biological functions. KS is
the least understood proteoglycan, which in part is due to a lack of panels of well-
defined KS oligosaccharides that are needed for structure-binding studies, as
analytical standards, to examine substrate specificities of keratinases and for drug
development. Here, we report a biomimetic approach that makes it possible to install …
| • | 引用: Increased expression of highly sulfated keratan sulfate synthesized … |
[PDF] Steviol Rebaudiosides bind to four different sites of the human sweet taste receptor (T1R2/T1R3) complex explaining confusing experiments
S Hao, B Guthrie, SK Kim, S Balanda, J Kubicek… - 2024
Sucrose provide sweetness and energy when it binds to both the Venus fly trap
domains (VFD) of heterodimeric sweet taste receptor (T1R2/T1R3), 1 while non-
caloric sweeteners (Sucralose, Aspartame, Neotame, Saccharin, Acesulfame-K (Ace-
K), Suosan, SC-45647, Fructose, and D-Tryptophan) bind only at the VFD of T1R2
(VFD2) to provide high-intensity sweetness. 2 Here, we address the binding
mechanism of various steviol glycosides (Rebaudioside B (RebB), RebM, RebD …
domains (VFD) of heterodimeric sweet taste receptor (T1R2/T1R3), 1 while non-
caloric sweeteners (Sucralose, Aspartame, Neotame, Saccharin, Acesulfame-K (Ace-
K), Suosan, SC-45647, Fructose, and D-Tryptophan) bind only at the VFD of T1R2
(VFD2) to provide high-intensity sweetness. 2 Here, we address the binding
mechanism of various steviol glycosides (Rebaudioside B (RebB), RebM, RebD …
| • | 引用: Structural basis for perception of diverse chemical substances by … |