Sugar cane molasses powder study of polyunsatura

Microbial fermentation of sugar cane molasses powder study of polyunsaturated fatty acids, in addition to PUFAs also be used as food additives, nutritional supplements, health care products and other accessories as well. Microbial nutrition has simple, growth and reproduction of fast, easy variation, the advantages of large-scale industrialized culture can determine a good way to use microbial production of oil is a development of new oil sources. At present, domestic and foreign studies often use glucose, starch and sucrose as a carbon source, such as the production of polyunsaturated fatty acids. The author attempts to filter out sugarcane molasses powder as a carbon source, fermentation production of polyunsaturated fatty acids from the soil strain, and strain mutation breeding, fermentation conditions, metabolic regulation, oil extraction and separation enrichment and other aspects of research, and oils by gas chromatography analysis of the composition and content identification.

The results of this study are as follows: 1, the use of Sudan Black B staining and polyunsaturated fatty acids, iodine value determined by a method selected from the soil strain of sugar cane molasses powder as raw material for the production of polyunsaturated fatty acids mold LB1. LB1 strain on morphological, physiological characteristics analysis and 5.8S rDNA internal transcribed on both sides of the gap gene sequence analysis concluded that the strain was recurvation Mucor (Mucor recurvus). 2, anti-soil screened starting flexor strain Mucor LB1, by ~ (60) Co-radiative recombination LiCl mutagenesis, were rich in polyunsaturated fatty acids Strains. Strains mainly in fat content and low molasses powder iodine value as the basis for selecting strains screened to an unsaturated fatty acid producing strain MR-10, compared with the original strain of the total lipid content increased 35.42%, iodine value increased 36.56%, PUFAs increased 43.76%. The strains were screened for genetic stability tests showed that after five strains cultured turn, has a good genetic stability.

3, in recurvation Mucor LB1 experiments to optimize the fermentation conditions, effects of different carbon sources: Impact of glucose, sucrose, starch and fermentation of PDA, etc.. Focus on the determination of molasses powder the components. From the results of composition analysis and the molasses and the economic point of view, fermentative production of molasses as a carbon source of polyunsaturated fatty acids, and most preferably a concentration of 15% molasses.

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