Up to 200 new jobs will be created when it opens in Newton Mearns, East Renfrewshire.
The 25,000-sq-ft (2,323-sq-m) store will be the company's fourth and largest outlet in Scotland.
It will bring the total number of staff employed in Scotland by Waitrose and its owners, the John Lewis Partnership, to more than 2,650.
Vacancies will be advertised on the partnership's jobs website from Monday for two weeks only.
Waitrose's sourcing team are currently meeting local producers to add to the 300 Scottish lines already introduced to its stores in Glasgow and Edinburgh.
A uggs for cheap online "Meet the Buyer" event, organised by Scotland Food and Drink, will be held during March in Glasgow.
Waitrose currently has stores in Glasgow's west end and in Morningside and Comely Bank in Edinburgh.