QUANTITECT PROBE RT-PCR KIT PDF EDITOR >> READ ONLINE
We currently use the QIAGEN QuantiTect Probe RT-PCR kit (#204443). The setup shown below is for the QIAGEN kit. Note also that the volume of RNA added per reaction below is 10 ul but can be increased or decreased with the appropriate adjustment of total volume with water. QuantiTect Probe RT-PCR Kits enable sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes. The QuantiNova Probe RT-PCR Kit overcomes the need for optimization of reaction conditions, which can be tedious and time consuming. Our RT-PCR kits provide everything needed to perform reverse transcription followed by PCR analysis of the synthesized cDNA. Choose our Enhanced AMV kit for difficult experiments with low abundance transcripts or difficult secondary structures. Or if you're looking for something more convenient 2 QuantiTect SYBR Green RT-PCR Handbook 11/2003 Contents Kit Contents 4 Shipping and Storage 4 Technical Assistance 4 Product Use Limitations 5 Number of 50 µl reactions 204243 200 2x QuantiTect SYBR Green RT-PCR Master Mix, containing: 3 x 1.7 ml ¦ HotStarTaq DNA Polymerase Kit Contents Shipping and Storage Product Use Limitations Product Warranty and Satisfaction Guarantee Probes with a diameter of 10 mm or above require larger tubes. See page 10 for a list of suppliers of For quantitative real-time RT-PCR we recommend QIAGEN QuantiTect™ RT-PCR Kits. All reactions were run with TaqMan® Gene Expression Master Mix (Thermo Fisher) under standard cycling conditions. The first four assays were run on the 7900 Fast Real-Time PCR System (Thermo Fisher) and the final assay (Cy5) was run on the LightCycler® Qiagen QuantiTect Probe PCR Kit. Inquiry Describtion* +Add Attachment (File Format: Jpg, Gif, Png, PDF,Zip,Txt,doc or xls Max Size: 3MB) iTaq Universal One-Step RT-qPCR Kits provide high PCR efficiency in sensitive SYBR Green or probe assays, even with difficult targets and challenging samples. iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad). QuantiTect SYBR®Green RT-PCR Kit (Qiagen). Power SYBR®RNA-to-Ct Kit QuantiFast Probe PCR +ROX Vial Kit (400)*. For 400 x 25 µl reactions: 3 x 1.7 ml 2x Master Mix (without ROX dye), 210 µl ROX Dye Solution, 2 x 2 ml RNase-Free Water. QuantiTect Probe PCR Kit — for real-time PCR and two-step RT-PCR using sequence-specific probes. RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to the two other commonly used techniques for quantifying mRNA levels, Northern blot analysis and RNase protection assay, RT-PCR can be used to Alternatively, qRT-PCR miRNA detection kits and primer sets are commercially available, but are Here we describe and provide protocols for an end-point and real-time looped RT-PCR procedure. B. UPL probe assay for miR166. No fluorescence was detected in the negative control reactions after
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