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Samples were amplified with primers EX1_F and EX1_R. The PCR products were digested overnight at 50 with 5 U Bsa I (New England Biolabs) and run on a 2% agarose gel. Normal DNA remains undigested (size 672 bp), DNA with the insertion is digested to 270 and 402 bp fragments.. ^^^ I agree with the above, it might help if you leave the house for a little bit every day. Go for a walk when he comes home, it doesn't have to be for long but they need time to build their own relationship. This is a very normal stage, and the best way to get past it is to just keep making it clear you aren't the only parent in the ghd gold mini styler house.